User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/06: Difference between revisions

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'''Line item 1'''<br>
> Samples


{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
 
|-valign="top"
{| {{table}}
| <u>Reagent</u> || <u>Volume</u>
|-
| Gene || Chromosome || Expected state || H3K27me3 max. DOC || H3K27me3 mock max. DOC || PcTF max. DOC || PcTF mock max. DOC
|-
| ALAS1 || chr 3 || active || ~160 ||  ||  ||  ||
|-
|-
| reagent 1 || # μL
| TBP || chr 6 || active || ~160 ||
|-
|-
| reagent 2 || #
| ACTB || chr 7 || active || ~180
|-
|-
| reagent 3 || #
| GUSB || chr 7 || active || ~160
|-
|-
| reagent 4 || #
| PPIA || chr 7 || active || ~90
|-
|-
| dH<sub>2</sub>O || #
| GAPDH || chr 12 || active || ~90
|-
|-
| &nbsp; || # μL
| B2M || chr 15 || active || 0
|-
| G6PD || chr X || active || ~130
|-
| HPRT1 || chr X (24) || active || ~150
|-
| PGK1 ||  chr X (24) || active || ~240
|-
| CDKN2A || chr9 || silent (H3K27me3+) || ~90
|-
| MMP12 || chr11 || silent (H3K27me3+) || ~18
|-
| MYT1 || chr20 || silent (H3K27me3+) || '''~270'''
|-
| OLIG1 || chr21 || silent (H3K27me3+) || 0
|-
| OLIG2 || chr21 || silent (H3K27me3+) || ~130
|}
|}
--> Reaction conditions




Revision as of 18:09, 6 January 2013

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


01/06/13

  • ChIP seq: run SeqMan NGen alignment on H3K27me3 mock (lane 7)
  • Expand table from 1/05/13 to include PcTF, PcTF mock, H3K27me3, H3K27me3 mock


Gene Chromosome Expected state H3K27me3 max. DOC H3K27me3 mock max. DOC PcTF max. DOC PcTF mock max. DOC
ALAS1 chr 3 active ~160
TBP chr 6 active ~160
ACTB chr 7 active ~180
GUSB chr 7 active ~160
PPIA chr 7 active ~90
GAPDH chr 12 active ~90
B2M chr 15 active 0
G6PD chr X active ~130
HPRT1 chr X (24) active ~150
PGK1 chr X (24) active ~240
CDKN2A chr9 silent (H3K27me3+) ~90
MMP12 chr11 silent (H3K27me3+) ~18
MYT1 chr20 silent (H3K27me3+) ~270
OLIG1 chr21 silent (H3K27me3+) 0
OLIG2 chr21 silent (H3K27me3+) ~130



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