Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/27"

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(Autocreate 2012/12/27 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
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==mm/dd/yy==
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==12/27/12==
 
<!-- Precede finished items with a checkmark &#x2713; -->
 
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
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* PcTF transfection: SK-N-SH TRIzol prep
  
 
----
 
----
'''Line item 1'''<br>
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'''SK-N-SH TRIzol prep'''<br>
> Samples
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# SK-N-SH, 1, +DNA
 
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# SK-N-SH, 2, (-)
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
 
|-valign="top"
 
| <u>Reagent</u> || <u>Volume</u>
 
|-
 
| reagent 1 || # μL
 
|-
 
| reagent 2 || #
 
|-
 
| reagent 3 || #
 
|-
 
| reagent 4 || #
 
|-
 
| dH<sub>2</sub>O || #
 
|-
 
| &nbsp; || # μL
 
|}
 
 
 
--> Reaction conditions
 
  
 +
* Discarded growth medium
 +
* Added 500 μL TRIzol directly to wells
 +
* incubated at r.t. for ~5 min.
 +
* Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
 +
* Transferred to 2.0 mL tubes. Stored at -80°C
  
  

Revision as of 16:05, 27 December 2012

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12/27/12

  • PcTF transfection: SK-N-SH TRIzol prep

SK-N-SH TRIzol prep

  1. SK-N-SH, 1, +DNA
  2. SK-N-SH, 2, (-)
  • Discarded growth medium
  • Added 500 μL TRIzol directly to wells
  • incubated at r.t. for ~5 min.
  • Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
  • Transferred to 2.0 mL tubes. Stored at -80°C