User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/27

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12/27/12

  • PcTF transfection: SK-N-SH TRIzol prep

SK-N-SH TRIzol prep

  1. SK-N-SH, 1, +DNA
  2. SK-N-SH, 2, (-)
  • Discarded growth medium
  • Added 500 μL TRIzol directly to wells
  • incubated at r.t. for ~5 min.
  • Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
  • Transferred to 2.0 mL tubes. Stored at -80°C