Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/26"

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==mm/dd/yy==
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==12/26/12==
 
<!-- Precede finished items with a checkmark &#x2713; -->
 
<!-- Precede finished items with a checkmark &#x2713; -->
 
* ChIP-seq enrichment: PcTF vs. PcTF mock for select genes
 
* ChIP-seq enrichment: PcTF vs. PcTF mock for select genes
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* Completed PcTF (lane 1) and PcTF mock (lane 2) BAM --> assembly workflow
 
* Completed PcTF (lane 1) and PcTF mock (lane 2) BAM --> assembly workflow
 
* Open in SeqMan pro to see raw values for four genes from 2011 paper.
 
* Open in SeqMan pro to see raw values for four genes from 2011 paper.
 +
 +
Workflow
 +
* Go to UCSC genome browser. Search for gene. Get coordinates.
  
 
1. CDKN2A,  
 
1. CDKN2A,  
  
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
 
|-valign="top"
 
| <u>Reagent</u> || <u>Volume</u>
 
|-
 
| reagent 1 || # μL
 
|-
 
| reagent 2 || #
 
|-
 
| reagent 3 || #
 
|-
 
| reagent 4 || #
 
|-
 
| dH<sub>2</sub>O || #
 
|-
 
| &nbsp; || # μL
 
|}
 
 
--> Reaction conditions
 
  
  

Revision as of 15:41, 26 December 2012

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12/26/12

  • ChIP-seq enrichment: PcTF vs. PcTF mock for select genes

ChIP: PcTF vs. PcTF mock

  • Completed PcTF (lane 1) and PcTF mock (lane 2) BAM --> assembly workflow
  • Open in SeqMan pro to see raw values for four genes from 2011 paper.

Workflow

  • Go to UCSC genome browser. Search for gene. Get coordinates.

1. CDKN2A,