User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18: Difference between revisions

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(Autocreate 2012/12/18 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
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==mm/dd/yy==
==12/18/12==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C
 


----
----
'''Line item 1'''<br>
'''TRIzol-RNeasy prep'''<br>
> Samples
# "K562 1": 1:2 dilution cells, +DNA
 
# "K562 1": 1:2 dilution cells, no DNA
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
# "K562 1": 1:4 dilution cells, +DNA
|-valign="top"
# "K562 1": 1:4 dilution cells, no DNA
| <u>Reagent</u> || <u>Volume</u>
# "K562 1": 1:8 dilution cells, +DNA
|-
# "K562 1": 1:8 dilution cells, no DNA
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}
 
--> Reaction conditions


* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
* Pellet cells at r.t./ 5 min./ 3000 rpm
* Discard supernatant
* Resuspend pellet in 500 μL TRIzol
* Store at -80°C for next steps (Carly)




Revision as of 15:34, 18 December 2012

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12/18/12

  • TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C


TRIzol-RNeasy prep

  1. "K562 1": 1:2 dilution cells, +DNA
  2. "K562 1": 1:2 dilution cells, no DNA
  3. "K562 1": 1:4 dilution cells, +DNA
  4. "K562 1": 1:4 dilution cells, no DNA
  5. "K562 1": 1:8 dilution cells, +DNA
  6. "K562 1": 1:8 dilution cells, no DNA
  • Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
  • Pellet cells at r.t./ 5 min./ 3000 rpm
  • Discard supernatant
  • Resuspend pellet in 500 μL TRIzol
  • Store at -80°C for next steps (Carly)



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