Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/12"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
 
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Latest revision as of 21:19, 26 September 2017

Owwnotebook icon.pngPc-TF Genomics Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


12/12/12

  • U2OS: split cells (passage 3)
  • SK-N-SH: split cells (passage 3) and set up transfection plate



U2OS

  • Split cells 1:10, 2x 75 cm flasks



SK-N-SH

  • Split cells 1:5, 1x 75 cm flask
  • Seed 6-well plate for transfection, ~5x105 cells/well/1 mL
    • Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well