Difference between revisions of "User:Karmella Haynes/Notebook/PcTF Genomics"

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==Illumina ChIP-seq==
 
==Illumina ChIP-seq==
Goals:
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* ChIP DNA prep for Illumina: [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2011/12/13 12/13/11],  [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2011/12/14 12/14/11], [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2011/12/19 12/19/11]
* Map Pc-TF binding & map H3K27me3 in U2OS
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* Fasta --> BAM files: Mark Teng
 +
* Peak finding DNASTAR Array Star [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/01 21/01/12]
 +
* Enrichment analysis (DNASTAR software)
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** BAM --> peaks/ enrichment histograms workflow [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/13 12/13/2012]
  
 
<br>
 
<br>
  
==Various Cell types==
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==Cell Type Study==
 
* Express PcTF (KAH126) in K-562 and SK-N-SH
 
* Express PcTF (KAH126) in K-562 and SK-N-SH
  

Revision as of 18:09, 7 January 2013

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Illumina ChIP-seq

  • ChIP DNA prep for Illumina: 12/13/11, 12/14/11, 12/19/11
  • Fasta --> BAM files: Mark Teng
  • Peak finding DNASTAR Array Star 21/01/12
  • Enrichment analysis (DNASTAR software)
    • BAM --> peaks/ enrichment histograms workflow 12/13/2012


Cell Type Study

  • Express PcTF (KAH126) in K-562 and SK-N-SH


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