User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base: Difference between revisions
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# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size | # BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size | ||
# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size | # BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size | ||
* Digests (Fermentas FD) | * Digests (Fermentas FD) | ||
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* Measure conc.'s | |||
{| {{table}} cellspacing="3" <!-- [DNA] table --> | {| {{table}} cellspacing="3" <!-- [DNA] table --> | ||
|- | |- bgcolor=#cfcfcf | ||
| Sample || OD260 || 260/280 || ng/μL | |||
| | |||
| | |||
| | |||
|- | |- | ||
| 1. Digested part (a/b) || --- || --- || --- | | 1. Digested part (a/b) || --- || --- || --- | ||
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* Dephosphorylation (Roche) | |||
{| {{table}} cellspacing="3" <!-- Dephos table --> | {| {{table}} cellspacing="3" <!-- Dephos table --> | ||
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* Ligations | |||
{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf |
Revision as of 14:35, 1 December 2012
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
mm/dd/yy
Minipreps
Assemblies
Oligo annealing
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