User:Karmella Haynes/Notebook/BioBrick cloning/2013/08/29: Difference between revisions
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'''Golden | '''Golden Gate''' | ||
* | * Try to increase robustness by using more T4 ligase | ||
# hPCD + BL01 + pSB1A3 | |||
# pSB1A3 alone (neg ctrl) | |||
* GG-PCR fragments generated on 1/10/13 | |||
** Diluted to 20 fmol/μL | |||
Golden Gate Reactions | Golden Gate Reactions | ||
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Thermal cycling | GG Thermal cycling | ||
* [45°C, 2 min.; 16°C, 5 min.] x25 | * [45°C, 2 min.; 16°C, 5 min.] x25 | ||
* 60°C, 20 min. | * 60°C, 20 min. |
Revision as of 13:27, 29 August 2013
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08/29/13
Golden Gate
Golden Gate Reactions
GG Thermal cycling
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