Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2013/04/18"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==04/18/13==
 
==04/18/13==
 
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* MV9 cloning - sequencing orer
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* MV9 cloning - sequencing order
  
  
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| Sample || OD260 || 260/280 || ng/μL
 
| Sample || OD260 || 260/280 || ng/μL
 
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| 1. Digested part (a/b) || --- || --- || ---
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| 1. 1-3 || 0.054 || 1.825 || 54.37
 
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| 2. Digested part (c/d) || --- || --- || ---
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| 2. 3-5 || 0.047 || 1.73 || 47.14
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| 3. 3-6 || 0.131 || 1.87 || 130.79
 
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* Sequencing results for order# 7177
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** MV9 1-3/ DD122B (fwd): correct sequence and orientation; reads through DD123B (rev primer) on other side
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** MV9 3-5/ DD122B (fwd): correct sequence and orientation; reads through DD123B (rev primer) on other side
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** MV9 3-6/ DD122B (fwd): correct sequence but backwards; reads through DD123B (rev primer) on other side
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* Keep MV9 1-3 in vector stocks. See [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2013/04/12 colony PCR from 04/12/13] for gel confirmation.
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* Keep MV9 3-6 in case it might be useful
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Latest revision as of 22:39, 26 September 2017

Owwnotebook icon.pngKarmella's BioBrick Cloning Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

04/18/13

  • MV9 cloning - sequencing order



MV9 sequencing

  • DNASU
    • DNA: 200 ng/ 10 μL
    • Primers: 16 pmol (16 μM) / 10 μL


  • Measure conc.'s
Sample OD260 260/280 ng/μL
1. 1-3 0.054 1.825 54.37
2. 3-5 0.047 1.73 47.14
3. 3-6 0.131 1.87 130.79


  • Sequencing results for order# 7177
    • MV9 1-3/ DD122B (fwd): correct sequence and orientation; reads through DD123B (rev primer) on other side
    • MV9 3-5/ DD122B (fwd): correct sequence and orientation; reads through DD123B (rev primer) on other side
    • MV9 3-6/ DD122B (fwd): correct sequence but backwards; reads through DD123B (rev primer) on other side


  • Keep MV9 1-3 in vector stocks. See colony PCR from 04/12/13 for gel confirmation.
  • Keep MV9 3-6 in case it might be useful