User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/20: Difference between revisions
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{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 || | ||
|- | |- | ||
| Insert DNA || 0.5 || --- || | | Insert DNA || 0.5 || 1.0 || 2.0 || --- || 0.5 || 1.0 || 2.0 || --- || | ||
|- | |- | ||
| Vector DNA || 1.0 || 1.0 || | | Vector DNA || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 || | | 2x lgn buf (Roche) || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || 5.0 || | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || 1.0 || | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 2.5 || 2.0 || 1.5 || 3.0 || 2.5 || 2.0 || 1.5 || 3.0 || | ||
|- | |- | ||
| || 10 μL || 10 μL || | | || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || 10 μL || | ||
|} | |} | ||
Revision as of 18:05, 20 March 2013
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03/20/13
Assemblies
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