User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/19: Difference between revisions
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==03/19/13== | ==03/19/13== | ||
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* Oligo annealing for new mammalian vector | * Oligo annealing for new mammalian vector | ||
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* Note: made fresh 10x annealing buffer | * Note: made fresh 10x annealing buffer | ||
# | # KAH3/MV2(X) + K-XbaI-NLS-his-stop | ||
* K-XbaI-NLS- | * K-XbaI-NLS-6his-st 1 | ||
* K-XbaI-NLS- | * K-XbaI-NLS-6his-st 2 | ||
* K-XbaI-NLS- | * K-XbaI-NLS-6his-st 3 | ||
* K-XbaI-NLS- | * K-XbaI-NLS-6his-st 4 | ||
{| | {| {{table}} <!-- Oligo annealing rxn table --> | ||
| DNA (oligos, 100 μM) || 4x 3 μL ea. = 12.0 | | DNA (oligos, 100 μM) || 4x 3 μL ea. = 12.0 | ||
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* 25°C, ∞ | * 25°C, ∞ | ||
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Revision as of 14:17, 22 March 2013
 Karmella's BioBrick Cloning
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03/19/13
Oligo annealing
Thermal Cycling (try new thermal cycling approach)
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