User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/06

From OpenWetWare
< User:Karmella Haynes‎ | Notebook‎ | BioBrick cloning‎ | 2013‎ | 03
Revision as of 16:57, 7 March 2013 by Karmella Haynes (talk | contribs) (03/06/13)
Jump to: navigation, search
Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

03/06/13

  • Colony PCR: for Rene's project



Colony PCR

  • Streaked single colonies on gridded plates yesterday
  • Used bit of streak for PCR today
Reagent Volume Expected:
Primers add ~250bp
1-3. BB 1 (# bp) = ##
4-6. R007.. (# bp) = ##
7. K.. (# bp) = ##
PCR gel
10 μL/lane; 1% agarose; Ladder
Bacteria from tip ---
10 μM VF2 primer 1.0
10 μM VR primer 1.0
2x GoTaq Green 12.5
dH2O 10.5
  25.0

PCR

  • 95°C, 3 min
  • [95°C, 15 sec; 57°C 15 sec; 72 °C 60 sec] x35
  • 72°C, 3 min
  • 4°C, ∞