Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/06"

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(mm/dd/yy)
(03/06/13)
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| bgcolor=#cfcfcf | Volume  
 
| bgcolor=#cfcfcf | Volume  
 
| rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
 
| rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
+
| rowspan="7" | [[Image:KAH030713_gel1.jpg|250px|PCR gel]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
 
|-
 
|-
 
| Bacteria from tip || ---
 
| Bacteria from tip || ---

Revision as of 17:55, 7 March 2013

Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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03/06/13

  • Colony PCR: for Rene's project



Colony PCR

  • Streaked single colonies on gridded plates yesterday
  • Used bit of streak for PCR today
Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
PCR gel
10 μL/lane; 1% agarose; Ladder
Bacteria from tip ---
10 μM VF2 primer 1.0
10 μM VR primer 1.0
2x GoTaq Green 12.5
dH2O 10.5
  25.0

PCR

  • 95°C, 3 min
  • [95°C, 15 sec; 57°C 15 sec; 72 °C 60 sec] x35
  • 72°C, 3 min
  • 4°C, ∞