User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/06: Difference between revisions
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== | ==03/06/13== | ||
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* | * Colony PCR: for Rene's project | ||
---- | ---- | ||
''' | '''Colony PCR'''<br> | ||
* | * Streaked single colonies on gridded plates yesterday | ||
* Used bit of streak for PCR today | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br> | | rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br> | ||
| rowspan="7" | | | rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| | | Bacteria from tip || --- | ||
|- | |- | ||
| | | 10 μM VF2 primer || 1.0 | ||
|- | |- | ||
| | | 10 μM VR primer || 1.0 | ||
|- | |- | ||
| | | 2x GoTaq Green || 12.5 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 10.5 | ||
|- | |- | ||
| || | | || 25.0 | ||
|} | |} | ||
PCR | |||
* 95°C, 3 min | |||
* [95°C, 15 sec; 57°C 15 sec; 72 °C 60 sec] x35 | |||
* 72°C, 3 min | |||
* 4°C, ∞ | |||
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Revision as of 17:54, 7 March 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||
03/06/13
Colony PCR
PCR
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