User:Karmella Haynes/Notebook/BioBrick cloning/2013/02/23: Difference between revisions
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Line 95: | Line 95: | ||
| Reagent || 1 || 2 || Master mix (x3) || 3 || 4 || Master mix (x3) | | Reagent || 1 || 2 || Master mix (x3) || 3 || 4 || Master mix (x3) | ||
|- | |- | ||
| | | pSB1A3 gg || 1.0 || 1.0 || --- || 2.0 || 2.0 || --- | ||
|- | |- | ||
| | | H2B gg || 1.0 || H2O || --- || 2.0 || H2O || --- | ||
|- | |- | ||
| | | LOV+his gg || 1.0 || H2O || --- || 2.0 || H2O || --- | ||
|- | |- | ||
| 10x PRO ligase buffer || 1.0 || 1.0 || 3.0 || 1.0 || 1.0 || 3.0 | | 10x PRO ligase buffer || 1.0 || 1.0 || 3.0 || 1.0 || 1.0 || 3.0 | ||
Line 111: | Line 111: | ||
| || 10.0 || 10.0 || || 10.0 || 10.0 || | | || 10.0 || 10.0 || || 10.0 || 10.0 || | ||
|} | |} | ||
* | * For 1 and 2, aliquot 7.0 mix to each tube; add DNA/ H2O | ||
* For 3 and 4, aliquot 4.0 mix to each tube; add DNA/ H2O | |||
Thermal cycling | Thermal cycling |
Revision as of 13:22, 25 February 2013
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02/23/13
Type IIS Assembly, PCR: LOV-H2B+his
PCR
Golden Gate Reactions
Thermal cycling
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