User:Karmella Haynes/Notebook/BioBrick cloning/2013/02/23: Difference between revisions
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| bgcolor=#cfcfcf | ng/μL | | bgcolor=#cfcfcf | ng/μL | ||
|- | |- | ||
| 1. H2B | | 1. H2B || 0.04 || 1.667 || 40.4 | ||
|- | |- | ||
| 2. LOV || 0.019 || 1.562 || 18.6 | | 2. LOV+his || 0.019 || 1.562 || 18.6 | ||
|- | |- | ||
| 3. pSB1A3 || 0.069 || 1.754 || 69.4 | | 3. pSB1A3 || 0.069 || 1.754 || 69.4 | ||
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| gg2 pSB1A3 || 1.0 || 1.0 || --- || 2.0 || 2.0 || --- | | gg2 pSB1A3 || 1.0 || 1.0 || --- || 2.0 || 2.0 || --- | ||
|- | |- | ||
| gg3 | | gg3 H2B || 1.0 || H2O || --- || 2.0 || H2O || --- | ||
|- | |- | ||
| gg4 | | gg4 LOV+his || 1.0 || H2O || --- || 2.0 || H2O || --- | ||
|- | |- | ||
| 10x PRO ligase buffer || 1.0 || 1.0 || 3.0 || 1.0 || 1.0 || 3.0 | | 10x PRO ligase buffer || 1.0 || 1.0 || 3.0 || 1.0 || 1.0 || 3.0 |
Revision as of 12:24, 25 February 2013
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02/23/13
Type IIS Assembly, PCR: LOV-H2B+his
PCR
Golden Gate Reactions
Thermal cycling
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