User:Karmella Haynes/Notebook/BioBrick cloning/2013/02/23: Difference between revisions
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| 1. H2B+his || 0.04 || 1.667 || 40.4 | | 1. H2B+his || 0.04 || 1.667 || 40.4 | ||
|- | |- | ||
| 2. LOV || 0.019 || 1.562 || 18. | | 2. LOV || 0.019 || 1.562 || 18.6 | ||
|- | |- | ||
| 3. pSB1A3 || 0.069 || 1.754 || 69. | | 3. pSB1A3 || 0.069 || 1.754 || 69.4 | ||
|- | |- | ||
| 4. pSB1A3 || 0.085 || 1.817 || 85. | | 4. pSB1A3 || 0.085 || 1.817 || 85.5 | ||
|} | |} | ||
Dilutions: to final concentration of 20 fmol/μL | Dilutions: to final concentration of 20 fmol/μL (40 μL) | ||
{| {{table}} border="1" cellspacing="3" <!-- Dilution table --> | {| {{table}} border="1" cellspacing="3" <!-- Dilution table --> | ||
|- valign="top" | |- valign="top" | ||
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| bgcolor=#cfcfcf | + dH<sub>2</sub>O | | bgcolor=#cfcfcf | + dH<sub>2</sub>O | ||
|- | |- | ||
| pSB1A3 || 2000 || | | pSB1A3 || 2000 || 85.5 || --- || --- | ||
|- | |- | ||
| H2B+his || | | H2B+his || 396 || 40.4 || --- || --- | ||
|- | |- | ||
| LOV || | | LOV || 429 || 66.9 || --- || --- | ||
|} | |} | ||
Revision as of 18:15, 23 February 2013
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02/23/13
Type IIS Assembly, PCR: LOV-H2B+his
PCR
Thermal cycling
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