User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/16

From OpenWetWare
< User:Karmella Haynes‎ | Notebook‎ | BioBrick cloning‎ | 2013‎ | 01
Revision as of 19:07, 16 January 2013 by Karmella Haynes (talk | contribs) (mm/dd/yy)
Jump to: navigation, search
Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

01/16/13

  • Golden Gate trial 3: results
  • Cultures: golden gate trial 3, 8 total



Golden Gate assembly trial 3

  • None of the DH5α-Turbo quick transformations had any colonies
  • Promega and Roche ligation buffer trials gave ratios of ~5:1 (assembly : vector only)
  • Pick 4 colonies from the Promega BL21 plate (#2) and the Roche BL21 plate (#10)