Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/15"

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* Make Promega buffer from scratch. Test Promega and NEB formulas.
 
* Make Promega buffer from scratch. Test Promega and NEB formulas.
 +
* Follow same procedure as ###
 +
* Transform into ### cells
  
 
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Revision as of 14:49, 15 January 2013

Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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01/15/13

  • Golden gate assembly: test different ligase buffers



Golden Gate Assembly optimization

  • Test different buffers
Buffer Tris-HCl MgCl2 DTT ATP
10x Promega 300 mM (pH 7.8) 100 mM 100 mM 10 mM
10x NEB 500 mM (pH 7.5) 100 mM 100 mM 10 mM
2x Roche  ?  ?  ?  ?
  • Make Promega buffer from scratch. Test Promega and NEB formulas.
  • Follow same procedure as ###
  • Transform into ### cells