User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/15

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01/15/13

  • Golden gate assembly: test different ligase buffers


Golden Gate Assembly optimization

  • Test different buffers
Buffer Tris-HCl MgCl2 DTT ATP
10x Promega 300 mM (pH 7.8) 100 mM 100 mM 10 mM
10x NEB 500 mM (pH 7.5) 100 mM 100 mM 10 mM
2x Roche ? ? ? ?
  • Make Promega buffer from scratch. Test Promega and NEB formulas.


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