User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/15: Difference between revisions
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* | * Golden gate assembly: test different ligase buffers | ||
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''' | '''Golden Gate Assembly optimization'''<br> | ||
* | * Test different buffers | ||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| Buffer || Tris-HCl || MgCl<sub>2</sub> || DTT || ATP | |||
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| | |||
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|- | |- | ||
| | | 10x Promega || 300 mM (pH 7.8) || 100 mM || 100 mM || 10 mM | ||
|- | |- | ||
| | | 10x NEB || 500 mM (pH 7.5) || 100 mM || 100 mM || 10 mM | ||
|- | |- | ||
| | | 2x Roche || ? || ? || ? || ? || ? | ||
|} | |} | ||
Revision as of 15:46, 15 January 2013
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01/15/13
Golden Gate Assembly optimization
Assemblies
Oligo annealing
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