Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/07"

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'''Gibson Re-transformation Results'''<br>
 
'''Gibson Re-transformation Results'''<br>
 
* Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate
 
* Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate
* Streaks from yesterday:  
+
* Streaks from yesterday: streak from plate 3 (hPCD + BL01 + pSB1A3 1xGibson) showed no color, whereas streak from plate 7 (hPCD + BL01 + pSB1A3 1/4x Gibson), showed RFP expression. The pSB1A3 template carries RFP.
 +
* Grow 2x 2 mL cultures from plate 3 streak to investigate whether an insert replaced RFP.
  
  

Revision as of 13:25, 7 January 2013

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01/07/13

  • Gibson re-transformation: grow cultures



Gibson Re-transformation Results

  • Number of colonies did not improve. Only 1 colony on hPCD + BL01 + V0120 plate
  • Streaks from yesterday: streak from plate 3 (hPCD + BL01 + pSB1A3 1xGibson) showed no color, whereas streak from plate 7 (hPCD + BL01 + pSB1A3 1/4x Gibson), showed RFP expression. The pSB1A3 template carries RFP.
  • Grow 2x 2 mL cultures from plate 3 streak to investigate whether an insert replaced RFP.