User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/11: Difference between revisions
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* Digests (Fermentas FD) | * Digests (Fermentas FD) | ||
{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> | ||
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| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |- | ||
| 1. KAH11 (E/P) || | | 1. KAH11 (E/P) || 0.019 || 1.96 || 18.7 | ||
|- | |- | ||
| 2. C-His (E/P) || | | 2. C-His (E/P) || 0.047 || 1.82 || 46.8 | ||
|} | |} | ||
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* pSB1A3-RFP (retransformation from 12/10/12) | * pSB1A3-RFP (retransformation from 12/10/12) | ||
* Note: pellets are red | * Note: pellets are red | ||
* Digest 10 μL of each with X/S for Gibson cloning (Brady and Vi) | * Digest 10 μL of each with X/S for Gibson cloning (Brady and Vi) | ||
* Note: combined mini preps afterwards; concentration = 60 ng/μL | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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* Gel purification: combined the two slices | * Gel purification: combined the two slices | ||
** Concentration: 260 = , 260/280 = , '''ng/μL = ''' | ** Concentration: 260 = 0.015, 260/280 = 2.2, '''ng/μL = 15.1''' | ||
Revision as of 17:14, 11 December 2012
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12/11/12
Assemblies
Minipreps
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