Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01"

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| dH<sub>2</sub>O || 9.5
 
| dH<sub>2</sub>O || 9.5
 
|-
 
|-
| &nbsp; || 15 μL --> 37°C/ ~15 min.
+
| &nbsp; || 15.0 μL <br>37°C/ ~15 min.
 
|}
 
|}
  

Revision as of 12:58, 2 December 2012

Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)



Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
Hover name
15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid. Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15.0 μL
37°C/ ~15 min.