User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01: Difference between revisions

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{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
|-valign="top"
|-valign="top"
| bgcolor=#cfcfcf | <u>Reagent</u>
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | <u>Volume</u>
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. MV2 = 4529<br>2. KAH3/MV2-1 = 5280<br>3. KAH3/MV2-2 = 5280<br>
| rowspan="7" | <u>Expected:</u><br>1. MV2 = 4529<br>2. KAH3/MV2-1 = 5280<br>3. KAH3/MV2-2 = 5280<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]

Revision as of 14:22, 1 December 2012

Karmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)


Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
 Hover name
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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