User:Karlena L. Brown/Notebook/PVOH Research/2013/04/03: Difference between revisions

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(Autocreate 2013/04/03 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
 
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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Collect all DSC results from dry clay samples previously prepared on '''2/08/13'''  
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
* Run DSC sample analysis on dry clay samples previously prepared on '''3/29/13'''
'''(Actual Mass = 0.4934g)'''
* Remove all excess oil from controlled microsphere samples prepared on '''03/29/13'''
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* Run fluorescence analysis on all microsphere samples prepared '''02/20/13''' with Rhodamine 6G dye
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==DSC Results for Dry Clay Samples==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
 
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH  
==DSC Analysis: Mixed Microspheres==
* Heat was reduced when solutions began to boil too rapidly
'''DSC preparation and protocol involving instrumentation and usage can be found on 9/17/12 entry.'''
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Name'''
| align="center" style="background:#f0f0f0;"|'''Sample Mass (mg)'''
| align="center" style="background:#f0f0f0;"|'''Pan / Lid Mass (mg)'''
|-
| 50:50 PVOH 130K NaMT||3.12||50.01
|-
| 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR||2.29||50.17
|-
| 90:10 PVOH 130K Laponite||2.59||50.12
|-
| 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR||2.27||50.28
|-
| 90:10 PVOH 146K 110% CEC NaMT w/ DMHXLBR||2.65||50.11
|-
| 50:50 PVOH 146K 110% CEC NaMT w/ DMHXLBR||2.50||50.17
|}
 
==Fluorescence Analysis: Diffusion Testing 2==
'''RECALL DIFFUSION TESTING PROTOCOL ON 2/13/13'''
 
* Fluorescent samples run and completed for 2hr diffusion rate determinations:
  # Microsphere 90:10 PVOH 146K 50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>
  # Microsphere 90:10 PVOH 146K 110% CEC Laponite w/ DMHXLBR
  # Microsphere 90:10 PVOH 146K NaMT
  # Microsphere 50:50 PVOH 146K CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>
  # Microsphere 50:50 PVOH 146K 110% CEC Laponite w/ DMHXLBR
 
* Specific parameters used to analyze fluorescence of hydrogel samples containing Rhodamine 6G dye included the following:
    # Excitation: 480nm
    # Emission Range: 500-650nm
    # Excitation Slit Width: 10
    # Emission Slit Width: 10
    # Scan Speed: 1200
 
==Notes==




Revision as of 20:08, 8 April 2013

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OBJECTIVES

  • Collect all DSC results from dry clay samples previously prepared on 2/08/13
  • Run DSC sample analysis on dry clay samples previously prepared on 3/29/13
  • Remove all excess oil from controlled microsphere samples prepared on 03/29/13
  • Run fluorescence analysis on all microsphere samples prepared 02/20/13 with Rhodamine 6G dye

DSC Results for Dry Clay Samples

DSC Analysis: Mixed Microspheres

DSC preparation and protocol involving instrumentation and usage can be found on 9/17/12 entry.

Sample Name Sample Mass (mg) Pan / Lid Mass (mg)
50:50 PVOH 130K NaMT 3.12 50.01
90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR 2.29 50.17
90:10 PVOH 130K Laponite 2.59 50.12
90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR 2.27 50.28
90:10 PVOH 146K 110% CEC NaMT w/ DMHXLBR 2.65 50.11
50:50 PVOH 146K 110% CEC NaMT w/ DMHXLBR 2.50 50.17

Fluorescence Analysis: Diffusion Testing 2

RECALL DIFFUSION TESTING PROTOCOL ON 2/13/13

  • Fluorescent samples run and completed for 2hr diffusion rate determinations:
  # Microsphere 90:10 PVOH 146K 50% CEC NaMT w/ Bu3HdP+
  # Microsphere 90:10 PVOH 146K 110% CEC Laponite w/ DMHXLBR
  # Microsphere 90:10 PVOH 146K NaMT
  # Microsphere 50:50 PVOH 146K CEC NaMT w/ Bu3HdP+
  # Microsphere 50:50 PVOH 146K 110% CEC Laponite w/ DMHXLBR
  • Specific parameters used to analyze fluorescence of hydrogel samples containing Rhodamine 6G dye included the following:
    # Excitation: 480nm
    # Emission Range: 500-650nm
    # Excitation Slit Width: 10
    # Emission Slit Width: 10
    # Scan Speed: 1200

Notes


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