User:Karlena L. Brown/Notebook/PVOH Research/2013/03/29: Difference between revisions
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== | ==OBJECTIVES== | ||
* Prepare controlled microsphere samples – PVOH 130K and 146K | |||
* | * Finish decanting microsphere samples prepared '''03/22/13''' | ||
''' | * Dry microsphere samples rinsed with hexanes '''(03/27/13)''' in preparation for DSC and X-ray | ||
* | * Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing | ||
'''( | |||
* | |||
''' | ==PVOH 146K Prepared Microsphere Samples & Dye Preparations 4== | ||
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13''' | |||
''' | '''1μM Rhodamine 6G Dye Concentration (90:10)''' | ||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
* | 1μM (RG6)x 10mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 109μL | ||
* | |||
'''1μM Rhodamine 6G Dye Concentration (50:50)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 61μL | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''PVOH Type''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH Mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Safflower Oil Added (mL)''' | |||
|- | |||
| 130K||0.10400||20||35 | |||
|- | |||
| 140K||0.1022||20||35 | |||
|} | |||
==Notes== | |||
* Samples previously rinsed with hexanes '''03/27/13''' and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction. | |||
* Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt. | |||
* The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples. | |||
* For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions. | |||
Revision as of 19:06, 8 April 2013
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OBJECTIVES
PVOH 146K Prepared Microsphere Samples & Dye Preparations 4RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13 1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Notes
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