User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 NaMT 0.02530 0.02530 40 165 61
50:50 Laponite 0.02590 0.02500 40 165 61
90:10 NaMT 0.04140 0.01110 40 92 109
90:10 Laponite 0.04470 0.01100 40 92 109
50:50 110% CEC Laponite w/ DMHXLBR 0.02550 0.02500 40 165 61
90:10 110% CEC Laponite w/ DMHXLBR 0.04230 0.01150 40 92 109
50:50 50% CEC NaMT w/ Bu3HdP+ 0.02540 0.02500 40 165 61
90:10 50% CEC NaMT w/ Bu3HdP+ 0.04010 0.01050 40 92 109

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres