Difference between revisions of "User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22"

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(PVOH Film Preparations)
(OBJECTIVES)
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==OBJECTIVES==
 
==OBJECTIVES==
* Continue to clean, filter, and separate out the oil from the previously prepared microspheres on '''2/22/13'''
 
 
* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
 
* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
 +
 +
==PVOH 130K Prepared Microsphere Samples & Dye Preparations 2==
 +
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
 +
 +
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
 +
 +
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
 +
|-
 +
| 50:50||NaMT||0.02530||0.02530||40||165||61
 +
|-
 +
| 50:50||Laponite||0.02590||0.02500||40||165||61
 +
|-
 +
| 90:10||NaMT||0.04140||0.01110||40||92||109
 +
|-
 +
| 90:10||Laponite||0.04470||0.01100||40||92||109
 +
|-
 +
| 50:50||110% CEC Laponite w/ DMHXLBR||0.02550||0.02500||40||165||61
 +
|-
 +
| 90:10||110% CEC Laponite w/ DMHXLBR||0.04230||0.01150||40||92||109
 +
|-
 +
| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.02540||0.02500||40||165||61
 +
|-
 +
| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.04010||0.01050||40||92||109
 +
|}
 +
 +
==Notes==
 +
* Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
 +
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
 +
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
 +
 +
* Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
  
 
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4==
 
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4==

Revision as of 13:37, 24 March 2013

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 NaMT 0.02530 0.02530 40 165 61
50:50 Laponite 0.02590 0.02500 40 165 61
90:10 NaMT 0.04140 0.01110 40 92 109
90:10 Laponite 0.04470 0.01100 40 92 109
50:50 110% CEC Laponite w/ DMHXLBR 0.02550 0.02500 40 165 61
90:10 110% CEC Laponite w/ DMHXLBR 0.04230 0.01150 40 92 109
50:50 50% CEC NaMT w/ Bu3HdP+ 0.02540 0.02500 40 165 61
90:10 50% CEC NaMT w/ Bu3HdP+ 0.04010 0.01050 40 92 109

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres

PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 110% CEC NaMT w/ DMHXLBR 0.05010 0.05100 40 165 61
90:10 110% CEC NaMT w/ DMHXLBR 0.01070 0.09100 40 92 109