User:Karlena L. Brown/Notebook/PVOH Research/2013/03/08

From OpenWetWare
Jump to navigationJump to search
PVOH Research <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

OBJECTIVES

Hydrogel Pressure Testing Protocol (Straight Pippette)

  1. Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample
  2. Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette
  3. Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H2O enter into the pipette by squeezing rubber bulb
  4. Progressively squeeze the bulb in order to expel the 3mL of H2O and apply a pressure to the hydrogels – dispensing Rhodamine 6G dye (dye leaching)
  5. Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis

Microspheres: Separation & Vacuum Filtration Procedures

Hydrogel Pressure Samples Tested 3

RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
1 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1006
2 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1022
3 90:10 130K Laponite 0.1031
4 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1066
5 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1083
6 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1003
7 50:50 130K 110% CEC Laponite w/ DMHXLBR 0.1036
8 50:50 146K Laponite 0.1016
9 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
10 90:10 146K 110% CEC NaMT w/ DMHXLBR 0.1020
11 50:50 130K NaMT 0.1024
12 50:50 130K 50% CEC NaMT w/ Bu3HdP+ 0.1015
13 50:50 146K 110% CEC NaMT w/ DMHXLBR 0.0998
14 90:10 130K 50% CEC NaMT w/ Bu3HdP+ 0.1026

Notes


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karlena_L._Brown/Notebook/PVOH_Research/2013/03/08&oldid=685214"