User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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# After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | # After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | ||
# Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | # Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | ||
==Important Liquid Nitrogen Safety== | |||
* Liquid nitrogen is toxic colorless odorless liquid that freely disintegrates into a gas'''(MW: 28.00 g/mole)''' | |||
* Liquid nitrogen is a cryogenic liquid that readily and instantaneously freezes items | |||
* Avoid all liquid nitrogen skin contact by wearing gloves and goggles | |||
* Liquid nitrogen should be kept in a closed cap double barreled metal tin when not in use to inhibit inhalation and asphyxiation | |||
* When in use, liquid nitrogen should be carefully poured into a double barreled small open metal container -- only a small amount needed | |||
* If spilled, allow substance to evaporate readily -- do not use a paper towel to wipe up substance because it will instantaneously freeze | |||
* If spilled, avoid standing too close to the spill or inhaling fumes | |||
* Keep all spills of liquid nitrogen in a closed space | |||
* When immersing samples into liquid nitrogen, use beaker tongs while standing at a safe distance away from the solution | |||
* Limit the contact of liquid nitrogen on gloves or other body parts due to ability to cause severe frostbite | |||
* Wash hands after removing gloves that were in contact with liquid nitrogen | |||
==PVOH 146K Prepared Samples & Dye Preparations== | ==PVOH 146K Prepared Samples & Dye Preparations== |
Revision as of 22:15, 25 February 2013
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OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
Important Liquid Nitrogen Safety
PVOH 146K Prepared Samples & Dye Preparations1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Hydrogel Pressure Testing Protocol
Hydrogel Pressure Samples Tested
Notes
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