User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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# After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | # After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | ||
# Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | # Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | ||
==PVOH 146K Prepared Samples & Dye Preparations== | |||
'''1μM Rhodamine 6G Dye Concentration (90:10)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 109μL | |||
'''1μM Rhodamine 6G Dye Concentration (50:50)''' | |||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | |||
1μM (RG6)x 10mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 61μL | |||
==Hydrogel Pressure Testing Protocol== | ==Hydrogel Pressure Testing Protocol== |
Revision as of 00:06, 25 February 2013
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OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
PVOH 146K Prepared Samples & Dye Preparations1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL Hydrogel Pressure Testing Protocol
Hydrogel Pressure Samples Tested
Notes |