User:Karlena L. Brown/Notebook/PVOH Research/2013/02/08: Difference between revisions
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== | ==OBJECTIVES== | ||
* Continue preparing polyvinyl alcohol microspheres for experimental analysis | |||
* | * Dilute Rhodamine 6G dye solution down to 1μM concentration for prepared microspheres | ||
* Prepare DSC samples for polyvinyl alcohol microspheres created | |||
* | |||
* | |||
''' | ==Microsphere PVOH 146K 110% CEC Laponite w/ DMHXLBR Information== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Preparation of Microspheres using PVA MW 146,000-186,000''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Amount of PVA added(g)||0.9001 | |||
|- | |||
| Amount of Lamponite clay added(g)||0.1004 | |||
|- | |||
| Amount of cellulose acetate butyrate added(g)||0.6066 | |||
|- | |||
| Amount of GA added(mL)||0.75 | |||
|- | |||
| Amount of H2SO4 added(mL)||0.25 | |||
|} | |||
<br> | |||
''' | ==Rhodamine 6G Dye Dilution Calculations== | ||
'''1μM Rhodamine 6G Dye Concentration''' | |||
* | M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | ||
* | 1μM (RG6)x 25mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 271.74μL | ||
==DSC Analysis: Exchanged Clays & PVOH 146K Microspheres== | |||
'''DSC preparation and protocal involving instrumentation and usage can be found on 9/17/12 entry.''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Name''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample Mass (mg)''' | |||
| align="center" style="background:#f0f0f0;"|'''Pan / Lid Mass (mg)''' | |||
|- | |||
| NaMT||3.16||48.13 | |||
|- | |||
| 50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||2.90||48.91 | |||
|- | |||
| 110% CEC NaMT w/ DMHXLBR||3.01||50.14 | |||
|- | |||
| Laponite||3.06||50.18 | |||
|- | |||
| 110% CEC Laponite w/ DMHXLBR||3.08||49.52 | |||
|- | |||
| 90:10 PVOH 146K Laponite Dust||2.89||48.87 | |||
|- | |||
| 90:10 PVOH 146K Laponite Medium Clumps||2.96||48.89 | |||
|- | |||
| 90:10 PVOH 146K Laponite Large Clumps||3.07||48.18 | |||
|} | |||
==Notes== | |||
* After preparing the microspheres from the PVOH 146K Laponite, many of the spheres were not spherical. Large clumps of all sizes were produced rather than microspheres. | |||
* The microspheres prepared from PVOH 146K Laponite were rigid white crystalline structures with stacked layers. | |||
* Due to large sizes of the microspheres, they had to be ground up using a mortar and pestle before adding the 1μM rhodamine 6G dye solution. | |||
* Also DSC was taken of the different sizes of the microspheres to determine if the structure, function, and capability of the microspheres had changed due to extensive clumping. | |||
* When going through the process of preparing new microspheres from PVOH 146K 110% CEC Laponite w/ DMHXLBR, due to slowed vacuum filtration the sample was placed in a vial with the last solvent used for cleaning, methanol. | |||
* When freezing the new microsphere sample in liquid nitrogen, PVOH 146K 110% CEC Laponite w/ DMHXLBR, it was frozen in a medium of methanol and vacuum filtrated to remove the methanol residue. | |||
Revision as of 22:41, 10 February 2013
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OBJECTIVES
Microsphere PVOH 146K 110% CEC Laponite w/ DMHXLBR Information
Rhodamine 6G Dye Dilution Calculations1μM Rhodamine 6G Dye Concentration M1V1 = M2V2 1μM (RG6)x 25mL = (92μM)V2 V2 = 271.74μL DSC Analysis: Exchanged Clays & PVOH 146K MicrospheresDSC preparation and protocal involving instrumentation and usage can be found on 9/17/12 entry.
Notes
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