Difference between revisions of "User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07"

From OpenWetWare
Jump to: navigation, search
(UV-Vis Limit of Detection For Salts)
(UV-Vis Limit of Detection For Salts)
(2 intermediate revisions by the same user not shown)
Line 11: Line 11:
  
 
==UV-Vis Limit of Detection For Salts==
 
==UV-Vis Limit of Detection For Salts==
To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.  
+
'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED'''
 +
 
 +
To find the limit of detection for the 10mM salts (Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.  
  
 
'''Solution of 1.89 μM porphyrin'''
 
'''Solution of 1.89 μM porphyrin'''
Line 35: Line 37:
  
 
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
 
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
 
+
[[Image:Al2%28SO4%293.jpg]]
 
+
<br><br>
 +
[[Image:Fe2%28SO4%293.jpg]]
 +
<br><br>
 +
[[Image:FeSO4.jpg]]
 +
<br><br>
 +
[[Image:Na2SO4.jpg]]
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 13:42, 30 November 2012

Owwnotebook icon.png PVOH Research <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

OBJECTIVES

  • Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
  • Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED

To find the limit of detection for the 10mM salts (Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin

  (94.45 μM)(V1)=(1.89 μM)(10mL)
   V1 = 0.2mL of 94.45 μM porphyrin
Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL]
1.89 µM 1 mM 1 mM 1 mM 1 mM 9.9
1.89 µM 2 mM 2 mM 2 mM 2 mM 9.8
1.89 µM 5 mM 5 mM 5 mM 5 mM 9.5
1.89 µM 10 mM 10 mM 10 mM 10 mM 9

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations

Al2(SO4)3.jpg

Fe2(SO4)3.jpg

FeSO4.jpg

Na2SO4.jpg