User:Karlena L. Brown/Notebook/PVOH Research/2012/10/31: Difference between revisions
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==Films Examined using FTIR== | ==Films Examined using FTIR== | ||
'''RECALL FTIR PROCEDURAL PROTOCOL 9/17/12''' | '''RECALL FTIR PROCEDURAL PROTOCOL 9/17/12''' | ||
'''LIST OF FILMS FOR FTIR:''' | |||
#PVOH(22K) 1.0067g+1mL GA+1mL porphyrin crosslinked | |||
#PVOH(22K)+0.5mL GA+0.104g NaMT crosslinked spun 10 min CuSO4 prepared 10/17/2012 | |||
#PVOH(22K)+0.5mLGA+0.99mL porphyrin(no color on film )crosslinked prepared 10/19/2012 | |||
#PVOH(22K)+1mL GA+100%CEC crosslinked prepared 10/4/2012 | |||
#PVOH(22K)+1mL GA+1mL porphyrin crosslinked prepared 10/24/2012 | |||
#PVOH(22K)+0.5mL GA+1mL porphyrin crosslinked prepared 10/24/2012 #2 | |||
#PVOH(22K)+0.5mLGA+1mL porphyrin prepared crosslinked 10/26/2012 | |||
#PVOH(22K)+0.8wt% GA+100%CEC crosslinked spun 10 min CuSO4 prepared 10/26/2012 | |||
#PVOH(22K)+0.8wt% GA+50% CEC crosslinked spun 10 min CuSO4 prepared 10/17/2012 | |||
[[Image:FTIR.JPG]] | |||
==Ion Diffusion & Ion Selective Electrode Film Procedures== | ==Ion Diffusion & Ion Selective Electrode Film Procedures== | ||
# Cut small piece of PVOH 22K film containing 1mL glutaldehyde exchanged in CuSO<sub>4</sub> for 10 min | # Cut small piece of PVOH 22K film containing 1mL glutaldehyde exchanged in CuSO<sub>4</sub> for 10 min | ||
# Place small cut piece of PVOH 22K film in plastic double graduated cylinder apparatus | # Place small cut piece of PVOH 22K film in plastic double graduated cylinder apparatus | ||
# Close and seal plastic double graduated cylinder apparatus | # Close and seal plastic double graduated cylinder apparatus | ||
# Next, in one graduated cylinder column pour in ~ 20mL of HPLC Grade H<sub>2</sub>O | # Next, in one graduated cylinder column pour in ~ 20mL of HPLC Grade H<sub>2</sub>O | ||
# Then, in other graduated cylinder column pour in ~20mL of the varied ion concentrated solution | # Then, in other graduated cylinder column pour in ~20mL of the varied ion concentrated solution | ||
# Plug the electrodes into the SympHony meter and turn on the ion selective electrode instruments | # Plug the electrodes into the SympHony meter and turn on the ion selective electrode instruments | ||
# Then press the arrows until ISE (ion selective electrode) and μS/cm ( | # Then press the arrows until ISE (ion selective electrode) and μS/cm (microsiemen per centimeter) appear on the screen | ||
# Remove the electrodes from the storage solutions | # Remove the electrodes from the storage solutions | ||
# Rinse the electrodes with distilled H<sub>2</sub>O and thoroughly dry both with KimWipes | # Rinse the electrodes with distilled H<sub>2</sub>O and thoroughly dry both with KimWipes | ||
# Place each electrode directly into the graduated cylinder columns containing either the HPLC Grade H<sub>2</sub>O or the varied ion solution for analysis (one electrode per column) | # Place each electrode directly into the graduated cylinder columns containing either the HPLC Grade H<sub>2</sub>O or the varied ion solution for analysis (one electrode per graduated column) | ||
# Measure the total amount of solution available and present for each graduated cylinder column | # Measure the total amount of solution available and present for each graduated cylinder column | ||
# Let each solution and electrode equilibrate for a set period of time (between 2-5 minutes) and then press measure on each instrument | # Let each solution and electrode equilibrate for a set period of time (between 2-5 minutes) and then press measure on each instrument | ||
# Once the measurement is finalized, record the values observed for each solution | # Once the measurement is finalized, record the values observed for each solution | ||
== | '''Ensure the hole separating the two graduated cylinder columns is completely shielded and covered by the PVOH 22K film''' | ||
==Ion Diffusion Film Analysis: Varied Ion Solution== | |||
'''A crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO<sub>4</sub> solution was used for an Ion Selective Electrode Analysis.''' | '''A crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO<sub>4</sub> solution was used for an Ion Selective Electrode Analysis.''' | ||
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== | ==Ion Diffusion Film Analysis: HPLC Grade H<sub>2</sub>O Solution== | ||
'''A crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO<sub>4</sub> solution was used for an Ion Selective Electrode Analysis.''' | '''A crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO<sub>4</sub> solution was used for an Ion Selective Electrode Analysis.''' | ||
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==Notes== | ==Notes== | ||
* The varied ion concentrated solution contained the following ions: Chlorine (Cl<sup> | * The varied ion concentrated solution contained the following ions: Chlorine (Cl<sup>-</sup>), Bromine (Br<sup>-</sup>), and Iodine (I<sup>-</sup>). | ||
* μS/cm ( | * μS/cm (microsiemen per centimeter) is a conductivity unit of measurement that is the inverse of an Ohm (O). | ||
* Experimental readings regarding the Ion Selective Electrode Analysis were taken every 30 minutes; however, for the first reading and recording only a 10 min interval surpassed before a reading was taken. | * Experimental readings regarding the Ion Selective Electrode Analysis were taken every 30 minutes; however, for the first reading and recording only a 10 min interval surpassed before a reading was taken. | ||
* The final two readings germane to the Ion Selective Electrode Analysis for the PVOH 22K film containing 1mL glutaldehyde that was exchanged for 10 min with CuSO<sub>4</sub> were taken by Dr. Fox. | * The final two readings germane to the Ion Selective Electrode Analysis for the PVOH 22K film containing 1mL glutaldehyde that was exchanged for 10 min with CuSO<sub>4</sub> were taken by Dr. Fox. |
Revision as of 11:49, 30 November 2012
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OBJECTIVES
Furfural Film Preparation
(PVOH 22K Actual Mass = 1.0029g)
(Furfural Actual Mass = 0.3192g)
Films Examined using FTIRRECALL FTIR PROCEDURAL PROTOCOL 9/17/12 LIST OF FILMS FOR FTIR:
Ion Diffusion & Ion Selective Electrode Film Procedures
Ensure the hole separating the two graduated cylinder columns is completely shielded and covered by the PVOH 22K film Ion Diffusion Film Analysis: Varied Ion SolutionA crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO4 solution was used for an Ion Selective Electrode Analysis.
Ion Diffusion Film Analysis: HPLC Grade H2O SolutionA crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO4 solution was used for an Ion Selective Electrode Analysis.
Notes
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