# Difference between revisions of "User:Karlena L. Brown/Notebook/PVOH Research/2012/10/19"

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## OBJECTIVES

• Prepare new CuSO4 standards for Atomic Absorption
• Re-examine NaMT-CuSO4 exchanged filtrate solutions using Atomic Absorption
• Crosslink all porphyrin films prepared
• Prepare new crosslinking fixant solutions

## New CuSO4 Dilution Standards for Atomic Absorption

• Solution 0.1 ppm CuSO4
```  (20 ppm CuSO4)V1 = (0.1 ppm CuSO4)(12mL)
V1 = 0.6mL of 20 ppm CuSO4
```
• Solution 0.5 ppm CuSO4 requires
```  (20 ppm CuSO4)V1 = (0.5 ppm CuSO4)(12mL)
V1 = 1.2mL of 20 ppm CuSO4
```
• Solution 1 ppm CuSO4 requires
```  (20 ppm CuSO4)V1 = (1 ppm CuSO4)(12mL)
V1 = 3mL of 20 ppm CuSO4
```
• Solution 2 ppm CuSO4 requires
```  (20 ppm CuSO4)V1 = (2 ppm CuSO4)(12mL)
V1 = 6mL of 20 ppm CuSO4
```
• Solution 3 ppm CuSO4 requires
```  (20 ppm CuSO4)V1 = (3 ppm CuSO4)(12mL)
V1 = 9mL of 20 ppm CuSO4
```

Using the following concentrations(shown above), a calibration curve was prepared.

## Atomic Absorption NaMT-CuSO4 Exchanged Filtrate Solutions

 ' Concentration [ppm] Abs. [ ] BG ' 0.01 -0.00600 0.00200 0.05 -0.01160 0.00540 1 -0.00920 0.00150 2 0.11710 0.00470 3 0.20330 0.00330 Given Conc. [ppm] Abs. [ ] BG Conc. [ppm] 1 min NaMT-CuSO4 7.4 0.37690 0.00430 5.63990 10 0.47600 0.00620 7.01910 5 min NaMT-CuSO4 7.4 0.38080 0.00350 5.69420 10 0.48150 0.00600 7.09560 10 min NaMT-CuSO4 7.4 0.29540 0.00370 4.50580 10 0.37640 0.00480 5.63300 1 min 100%CEC NaMT-CuSO4 7.4 0.48030 0.00680 7.07890 10 0.61880 0.00950 9.00630 5 min 100%CEC NaMT-CuSO4 7.4 0.46120 0.00640 6.81310 10 0.62080 0.00790 9.03420 10 min 100%CEC NaMT-CuSO4 7.4 0.43300 0.00400 6.42070 10 0.62960 0.00480 9.15660 1 min 50%CEC NaMT-CuSO4 7.4 0.47470 0.00530 7.00100 10 0.62050 0.00490 9.03000 5 min 50%CEC NaMT-CuSO4 7.4 0.46110 0.00310 6.81170 10 0.60940 0.00580 8.87550 10 min 50%CEC NaMT-CuSO4 7.4 0.46800 0.00390 6.90770 10 0.59620 0.00440 8.69180

## Fixant Solution Preparation

1. Weigh out ~200g Na2SO4
``` (Actual Na2SO4 Mass: 199.9953g)
```
1. Add the Na2SO4 solids to a 1000mL closed cap plastic bottle
2. Next, add ~1000mL of H2O to the bottle along with large magnetic stir bar
3. Place container with the solution on a stir plate and stir solution for ~20 minutes or until all solids dissipate into solution
4. Add 20mL of concentrated H2SO4 to solution and continue stirring for ~10 minutes more