User:Karlena L. Brown/Notebook/PVOH Research/2012/10/17

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Revision as of 12:03, 23 November 2012 by Karlena L. Brown (talk | contribs) (UV - Vis Procedures)
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Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures

UV - Vis Procedures

  1. Open UV-Vis Pro program on the computer
  2. Hit the UV-Vis switch at the bottom of the computer setup
  3. On the bottom of computer screen, click connect button
  4. Go through UV-Vis Safety Connection Procedures and Protocols for ~ 15 min
  5. Once finished safety protocol run, click method button
  6. Select a instrument scanning range (wavelengths = 200 - 800nm)
  7. Click data window button to create new folder under → CHEM 581 → MKM
  8. Create name for sample run under new folder MKM
  9. Prepare a baseline (blank) sample to run in UV-Vis
  10. Use quartz cuvette specially made for UV-Vis and fill cuvette ¾ full with HPLC grade H2O
  11. Wipe quartz cuvette gently and thoroughly with a Kim Wipe
  12. Place cuvette in reference pocket of six sample UV-Vis instrument
  13. Approve selection and file name then click baseline button
  14. Run baseline of blank sample (HPLC grade H2O)
  15. Once finished running, autoscale the y-axis on the screen until max absorption = 1.0
  16. Save sample name under new folder MKM

Repeat procedure for all UV-Vis Samples run; however, do not have to run a baseline every time after each sample. Baseline only has to be run once throughout the total analysis. Also please be sure to clean each cuvette and wipe thoroughly each time before running new samples for analysis. In addition, place actual samples that are not references in the first pocket of six sample holder in UV-Vis.

UV-Vis of Porphyrin Solutions

Porphyrin Film Preparations