User:Karlena L. Brown/Notebook/PVOH Research/2012/10/17: Difference between revisions
Line 50: | Line 50: | ||
==Porphyrin Dilution Calculations== | ==Porphyrin Dilution Calculations== | ||
* Solution of 94.45 μM porphyrin | |||
(94.45 μM)(V<sub>1</sub>)=(9.445 μM)(10mL) | |||
V<sub>1</sub> = 1mL of 94.45 μM porphyrin | |||
* Solution of 9.445 μM porphyrin | |||
(9.445 μM)(V<sub>1</sub>)=(944.5 nM)(10mL) | |||
V<sub>1</sub> = 1mL of 9.445 μM porphyrin | |||
* Solution of 944.5 nM porphine | |||
(944.5 nM)(V<sub>1</sub>)=(94.45 nM)(10mL) | |||
V<sub>1</sub> = 1mL of 944.5 nM porphyrin | |||
* Solution of 944.5 nM porphyrin | |||
(944.5 nM)(V<sub>1</sub>)=(9.445 nM)(10mL) | |||
V<sub>1</sub> = 0.1mL of 944.5 nM porphyrin | |||
* Solution of 94.45 μM porphyrin | |||
(94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL) | |||
V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin | |||
* Solution of 94.45 uM porphyrin | |||
(94.45 μM)(V<sub>1</sub>)=(3.78 μM)(10mL) | |||
V<sub>1</sub> = 0.4mL of 94.45 μM porphyrin | |||
==UV-Vis of Porphyrin Solutions== | ==UV-Vis of Porphyrin Solutions== |
Revision as of 20:47, 23 November 2012
PVOH Research | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||
OBJECTIVES
Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration ProceduresFollow procedural protocol for Cu2+ film exchanged solutions & vacuum filtration procedures 10/05/12; however, instead of using clay, use 1.0g films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval.
UV - Vis Procedures
Porphyrin Dilution Calculations
(94.45 μM)(V1)=(9.445 μM)(10mL) V1 = 1mL of 94.45 μM porphyrin
(9.445 μM)(V1)=(944.5 nM)(10mL) V1 = 1mL of 9.445 μM porphyrin
(944.5 nM)(V1)=(94.45 nM)(10mL) V1 = 1mL of 944.5 nM porphyrin
(944.5 nM)(V1)=(9.445 nM)(10mL) V1 = 0.1mL of 944.5 nM porphyrin
(94.45 μM)(V1)=(1.89 μM)(10mL) V1 = 0.2mL of 94.45 μM porphyrin
(94.45 μM)(V1)=(3.78 μM)(10mL) V1 = 0.4mL of 94.45 μM porphyrin UV-Vis of Porphyrin Solutions
* Wavelength range = 200 - 800nm * Slit width = 1.0nm UV-Vis Analysis Notes
Porphyrin Film PreparationsFollow procedural protocol for preparing glutaldehyde films 9/25/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde for each film before pouring substance into a Teflon dish.
|