User:Kara Tsukashima/Notebook/Yogurt Lactobacillus RFLP identification/2013/05/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Picking colonies== | ||
Nine isolated colonies were streaked onto the plates made 27 April 2013. They were placed into the 37ºC incubator. | |||
== Observations == | |||
The "kinds" of colonies did not separate out so readily. However, it was noticed that some were umbonate and others flat--irregular edges on all but two. It was not terribly feasible to check for morphology on a stain given that the staining supplies were somewhere less than easily discerned. | |||
==Next Week== | |||
I will check for growth Monday, as previously at least some growth was visible by then. If it appears that at least a certain quantity are growing, I will prepare for PCR, digest and gel-running (buying primers, etc) on 11 May 2013. | |||
Latest revision as of 22:40, 26 September 2017
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Picking coloniesNine isolated colonies were streaked onto the plates made 27 April 2013. They were placed into the 37ºC incubator. ObservationsThe "kinds" of colonies did not separate out so readily. However, it was noticed that some were umbonate and others flat--irregular edges on all but two. It was not terribly feasible to check for morphology on a stain given that the staining supplies were somewhere less than easily discerned. Next WeekI will check for growth Monday, as previously at least some growth was visible by then. If it appears that at least a certain quantity are growing, I will prepare for PCR, digest and gel-running (buying primers, etc) on 11 May 2013.
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