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| ==Joe C. Liang== | | ==Joe C. Liang== |
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| | [[Image:Joe.jpg|right|thumb]] |
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| Ph.D. Candidate <br/> | | Ph.D. Candidate <br/> |
| Bioengineering -- [http://openwetware.org/wiki/Smolke Smolke Lab] <br/> | | Bioengineering -- [http://openwetware.org/wiki/Smolke Smolke Lab] <br/> |
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| 473 Via Ortega <br/> | | 473 Via Ortega <br/> |
| Stanford, CA 94305 <br/> | | Stanford, CA 94305 <br/> |
| Phone: (650) 721-6373 <br/> | | Phone: (650) 721-5884 <br/> |
| madonjoe @ stanford . edu
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| ==Education== | | ==Education== |
| Ph.D Student, Chemical Engineering, Caltech, 2006-present <br/> | | Ph.D, Candidate, Chemical Engineering, Caltech, 2008-present <br/> |
| M.S, Chemical Engineering, Caltech, 2008 <br/> | | M.S, Chemical Engineering, Caltech, 2008 <br/> |
| B.S, Chemical Engineering with Emphasis in Applied Physical Science, UC Berkeley, 2006<br/> | | B.S, Chemical Engineering, UC Berkeley, 2006<br/> |
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| ==Research Interest== | | ==Research Interest== |
| RNA aptamers are known to discriminate molecules in a highly sensitive way. One classic example is that the theophylline aptamer does not respond to caffeine though there is mere difference of one methyl group in structure. This superior sensitivity of aptamers distinguishing molecules is a nice characteristic to be incorporated in a synthetic biological regulatory network, which usually consists of many structurally similar metabolites. However, the application of using aptamers in a synthetic pathway has been severely limited by the ability to generate functional aptamers in vivo effectively. Traditional in vitro SELEX usually takes weeks in selection and characterization of aptamers and requires further screening for in vivo activities. In the Smolke lab, we have developed several portable, modular, and tunable engineered RNA-based switch platform that can adapt to different conformations upon binding of various molecular inputs and subsequently modulates the level of target gene expression. We can then use the existing sensor domain of these switch platforms developed from SELEX and implement it as a selection tool to screen library in order to generate aptamers for other structurally similar molecules. The eventual goal is to incorporate these platforms as part of the regulatory network in a synthetic biological pathway and redirect molecular fluxes to accumulate medicinal valuable intermediates that are too costly to be synthesized chemically.
| | I am developing enabling technologies that support generation of RNA devices. |
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| ==Publications== | | ==Publications== |
| <biblio>
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| #liang1 [http://www.cs.caltech.edu/cbsss/finalreport/yjunction_group.pdf ''Y-Junction Carbon Nanotube Implementation of Intramolecular Electronic NAND Gate'']
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| <br>Benjamin Gojman, Happy Hsin, Joe Liang, Natalia Nezhdanova, Jasmin Saini
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| #liang2 ''Evaluation of Two Computational Models Based on Different Effective Core Potentials for Use in Organocesium Chemistry''
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| <br>Streitwieser, A.; Liang, J. C.-Y.; Jayasree, E. G.; Hasanayn, F. J. Chem. Theory and Comput.; (Article); 2007; 3(1); 127-131
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| </biblio>
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| =Honors and Awards=
| | # Liang JC, Smolke CD. 2011. Rational design and tuning of ribozyme-based devices. In: Hartig J, editor. Methods in Molecular Biology. In press. |
| | # Win MN, Liang JC, Smolke CD. 2010. Frameworks for programming RNA devices. In: Mayer G, editor. The Chemical Biology of Nucleic Acids. U.K.: John Wiley & Sons, Ltd. pp. 323-38. |
