Difference between revisions of "User:Jhdavis"

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(Protocols)
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#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]]
 
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]]
 
#[[Sauer:Making_yeg_plates|YEG-Cl]]
 
#[[Sauer:Making_yeg_plates|YEG-Cl]]
 +
#[[Sauer:Davis Minimal Media]]
  
 
==Info==
 
==Info==

Revision as of 13:49, 1 May 2009

I recently joined the Sauer Lab. clpX holla!!!!

Protocols

  1. Western Blots by Eyal Jhdavis::Western::Eyal
  2. GFP Purification by organic extraction Jhdavis::GFPOrganic
  3. Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
  4. Peptide cleavage Jhdavis::PeptideCleavage
  5. Flp recombinase Jhdavis::Flping
  6. ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
  7. Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
  8. regenerating qiagen columns Jhdavis::regenQiagen
  9. atpase notes Jhdavis::atpaseNotes
  10. Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip

Buffers

  1. PD Buffer
  2. ATP Regeneration Mix
  3. YEG-Cl
  4. Sauer:Davis Minimal Media

Info

Links

  1. Promoter standard
  2. novagen plasmid compatibility

Sheets

  1. File:Asdfsa.xlsx