Difference between revisions of "User:Jhdavis"

From OpenWetWare
Jump to: navigation, search
(Buffers)
 
(3 intermediate revisions by the same user not shown)
Line 9: Line 9:
 
#Flp recombinase [[Jhdavis::Flping]]
 
#Flp recombinase [[Jhdavis::Flping]]
 
#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]]
 
#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]]
#Sumo Protease expression/purification from Malakhov 2004 [[Jhdaivs::SumoPrep]]
+
#Sumo Protease expression/purification from Malakhov 2004 [[Jhdavis::SumoPrep]]
 
#regenerating qiagen columns [[Jhdavis::regenQiagen]]
 
#regenerating qiagen columns [[Jhdavis::regenQiagen]]
 
#atpase notes [[Jhdavis::atpaseNotes]]
 
#atpase notes [[Jhdavis::atpaseNotes]]
 +
#Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) [[Jhdavis::Immunoprecip]]
  
 
==Buffers==
 
==Buffers==
Line 17: Line 18:
 
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]]
 
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]]
 
#[[Sauer:Making_yeg_plates|YEG-Cl]]
 
#[[Sauer:Making_yeg_plates|YEG-Cl]]
 +
#[[Sauer:Davis Minimal Media]]
 +
#[[Jhdavis::4x reducing SDS-PAGE loading buffer]]
  
 
==Info==
 
==Info==
Line 25: Line 28:
 
*[[Jhdavis::Useful pymol]]
 
*[[Jhdavis::Useful pymol]]
 
*[[Jhdavis::A260:A280 DNA:Protein ratios]]
 
*[[Jhdavis::A260:A280 DNA:Protein ratios]]
 +
*[[Jhdavis::fplc singup]]
  
 
==Links==
 
==Links==

Latest revision as of 11:26, 6 May 2009

I recently joined the Sauer Lab. clpX holla!!!!

Protocols

  1. Western Blots by Eyal Jhdavis::Western::Eyal
  2. GFP Purification by organic extraction Jhdavis::GFPOrganic
  3. Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
  4. Peptide cleavage Jhdavis::PeptideCleavage
  5. Flp recombinase Jhdavis::Flping
  6. ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
  7. Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
  8. regenerating qiagen columns Jhdavis::regenQiagen
  9. atpase notes Jhdavis::atpaseNotes
  10. Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip

Buffers

  1. PD Buffer
  2. ATP Regeneration Mix
  3. YEG-Cl
  4. Sauer:Davis Minimal Media
  5. Jhdavis::4x reducing SDS-PAGE loading buffer

Info

Links

  1. Promoter standard
  2. novagen plasmid compatibility

Sheets

  1. File:Asdfsa.xlsx