# Difference between revisions of "User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/03/05"

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## Entry title

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## Objective

Today we'll be calculating the equilibrium constants for heated 30:1 lysozyme AuNPs with variables added after baking, and room temperature 30:1 lysozyme AuNPs. Then we will make standards with the amount of gold per 5mL tube to run AA and UV-Vis. Then we will centrifuge the samples from yesterday, and run AA and UV-Vis on centrifuged samples.

## Procedure

### Atomic Absorption

Creating the Gold Stock Solutions

1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
6. A sixth stock solution of gold was created with 448 μL of HAuCl4·3H2O and 4552 μL of distilled water to a Falcon tube. This resulting concentration represents the amount of gold in a 5 mL sample of lysozyme-AuNP.

Atomic Absorption Samples

The following samples were run on the AA:

• 30:1 lysozyme-AuNP with NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid
• 60:1, 70:1, 80:1, 90:1, 100:1 BSA-AuNP with MgCl2
• Gold solution in 5 mL sample of lysozyme-AuNP
• Control of 30:1 lysozyme-AuNP without any added variable

## Figures

After running Atomic Absorbance, we performed data analysis on the results.

Figure 1. Atomic absorbance of Lysozyme with different concentrations of variables