User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/03/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 33: Line 33:
* Control of 30:1 lysozyme-AuNP without any added variable
* Control of 30:1 lysozyme-AuNP without any added variable


==Figures==
After running Atomic Absorbance, we performed data analysis on the results.
''Figure 1. Atomic absorbance of Lysozyme with different concentrations of variables''
[[]]




Revision as of 10:31, 21 March 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Insert content here...

Objective

Today we'll be calculating the equilibrium constants for heated 30:1 lysozyme AuNPs with variables added after baking, and room temperature 30:1 lysozyme AuNPs. Then we will make standards with the amount of gold per 5mL tube to run AA and UV-Vis. Then we will centrifuge the samples from yesterday, and run AA and UV-Vis on centrifuged samples.

Procedure

Atomic Absorption

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
  6. A sixth stock solution of gold was created with 448 μL of HAuCl4·3H2O and 4552 μL of distilled water to a Falcon tube. This resulting concentration represents the amount of gold in a 5 mL sample of lysozyme-AuNP.


Atomic Absorption Samples

The following samples were run on the AA:

  • 30:1 lysozyme-AuNP with NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid
  • 60:1, 70:1, 80:1, 90:1, 100:1 BSA-AuNP with MgCl2
  • Gold solution in 5 mL sample of lysozyme-AuNP
  • Control of 30:1 lysozyme-AuNP without any added variable

Figures

After running Atomic Absorbance, we performed data analysis on the results.

Figure 1. Atomic absorbance of Lysozyme with different concentrations of variables

[[]]



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Javier_Vinals_Camallonga/Notebook/Javier_Vinals_notebook/2014/03/05&oldid=782585"