User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/02/25: Difference between revisions

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==Entry title==
==Objective==
* Insert content here...
* Today we will be spinning down samples of 30:1 lysozyme Au-Np with 1M salts added, and then UV-Vis will be run on them. After this, the samples with the salts will be heated overnight at 37°C.
After this, we will image the results of the 30:1, 55:1, and 110:1 lysozyme Au-Nps synthesized with salt added before heating. Then we will run atomic absorption of 30:1, 55:1 and 110:1 lysozyme AuNPs synthesized with salts before heating.
 
==Procedure==
 
===Atomic Absorption===
 
 
'''Creating the Gold Stock Solutions'''
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
 
 
'''Atomic Absorption Samples'''
 
Solutions with the following Au:lysozyme ratio were run on the AA:
* 30:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl
* 55:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl
* 110:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl
 
 
==Images==
 
 
===Atomic Absorance of Lysozyme with KCl, NaCl, CaCl<sub>2</sub>, MgCl<sub>2</sub>===
 
''Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts''
 
[[Image:Lysozyme Gold-protein to Concentration with Salts, JAC.png|550px|]]
 
===UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP Synthesis===
[[Image:Screen_shot_2014-02-25_at_11.48.04_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.47.52_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.47.42_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.47.31_PM.png]]
 
===UV-Vis For Select Variables at More Refined Concentrations===
[[Image:Screen_shot_2014-02-25_at_11.47.14_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.46.46_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.46.17_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.45.14_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.45.00_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.44.28_PM.png]]
[[Image:Screen_shot_2014-02-25_at_11.44.01_PM.png]]
 
==Notes==
 
[[Image:notes.2.25.jac.png|700px|]]
 
These are the results that sum up the samples from last Wednesday with the addition of the variables to 1mL aliquots of 30:1 ratio with salts added after
 
[[Image:more.2.25.notes.png|500px|]]
 
These are the results detail the reaction of different ratios synthesized with salts before heating


==Image==
==Image==

Revision as of 16:05, 16 March 2014

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Objective

  • Today we will be spinning down samples of 30:1 lysozyme Au-Np with 1M salts added, and then UV-Vis will be run on them. After this, the samples with the salts will be heated overnight at 37°C.

After this, we will image the results of the 30:1, 55:1, and 110:1 lysozyme Au-Nps synthesized with salt added before heating. Then we will run atomic absorption of 30:1, 55:1 and 110:1 lysozyme AuNPs synthesized with salts before heating.

Procedure

Atomic Absorption

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio were run on the AA:

  • 30:1 MgCl2, CaCl2, NaCl, KCl
  • 55:1 MgCl2, CaCl2, NaCl, KCl
  • 110:1 MgCl2, CaCl2, NaCl, KCl


Images

Atomic Absorance of Lysozyme with KCl, NaCl, CaCl2, MgCl2

Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts

UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP Synthesis

UV-Vis For Select Variables at More Refined Concentrations

Notes

These are the results that sum up the samples from last Wednesday with the addition of the variables to 1mL aliquots of 30:1 ratio with salts added after

These are the results detail the reaction of different ratios synthesized with salts before heating

Image

Atomic Absorance of Lysozyme with KCl, NaCl, CaCl2, MgCl2

'Figure 1. Gold:protein ratio to Concentration'


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