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==Objective==
 
==Objective==
Today we'll be determining the molar absorptivities of two different molecules, [http://en.wikipedia.org/wiki/Adenosine adenosine] and [http://en.wikipedia.org/wiki/Inosine inosine]. The data that we generate today will be important when we study [http://en.wikipedia.org/wiki/Adenosine_deaminase adenosine deaminase] (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxy group. Overexpression of this protein causes anemia in humans. A shortage of this protein can lead to severe immuno-defficiency.  
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Today we'll be running 20 samples in the UV-Vis, 10 for Invertase and 10 for BSA. First of all, we centrifuged the samples for 30 minutes, and then we run UV-Vis spectra absorption on them. We obtained the following results:
  
Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. Just as each molecule has a characteristic absorption at each wavelength, this (per-wavelength) absorption can be quantified by a molar absorptivity. Or ... for a given concentration a molecule will absorb a very specific amount of light at a precise wavelength. A molecule doesn't have just one molar absorptivity; there is a molar absorptivity to describe each wavelength in a molecular absorbance spectrum.
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'Figure 1. Invertase Spectra absorption UV-Vis'
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[[Image:Invertase UV-Vis spectra Javier vinals.png]]
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'Figure 2. BSA Spectra absorption UV-Vis'
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[[Image:BSA sectra absorption UV-Vis Javier vinals .png]]
  
  

Latest revision as of 23:37, 26 September 2017

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Objective

Today we'll be running 20 samples in the UV-Vis, 10 for Invertase and 10 for BSA. First of all, we centrifuged the samples for 30 minutes, and then we run UV-Vis spectra absorption on them. We obtained the following results:

'Figure 1. Invertase Spectra absorption UV-Vis'

Invertase UV-Vis spectra Javier vinals.png

'Figure 2. BSA Spectra absorption UV-Vis'

BSA sectra absorption UV-Vis Javier vinals .png