Difference between revisions of "User:Jarle Pahr/Recipes"

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(New page: LB (1000 mL): 10 g tryptone 5 g yeast extract 5 g NaCl)
 
 
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LB (1000 mL):
 
LB (1000 mL):
 
10 g tryptone
 
10 g tryptone
 
5 g yeast extract
 
5 g yeast extract
 
5 g NaCl
 
5 g NaCl
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 +
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Terrfic Broth (http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8620.full?text_only=true):
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900 mL H2O
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12 g tryptone
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24 g yeast extract
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4 mL glycerol
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Autoclave and add 100 mL sterile solution 0.17 M KH2PO4, 0.72 M K2HPO4.
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0.17 :M KH2PO4, 0.72 M K2HPO4:
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2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 mL of H2O. Dissolve salts, adjust volume to 100 mL and autoclave.

Latest revision as of 05:48, 9 May 2013

LB (1000 mL): 10 g tryptone 5 g yeast extract 5 g NaCl


Terrfic Broth (http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8620.full?text_only=true):

900 mL H2O 12 g tryptone 24 g yeast extract 4 mL glycerol

Autoclave and add 100 mL sterile solution 0.17 M KH2PO4, 0.72 M K2HPO4.

0.17 :M KH2PO4, 0.72 M K2HPO4:

2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 mL of H2O. Dissolve salts, adjust volume to 100 mL and autoclave.