Difference between revisions of "User:Jarle Pahr/Promoters"

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=Reporter systems=
 
=Reporter systems=
  
http://www.weizmann.ac.il/mcb/UriAlon/index.html
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http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html
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(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html )
  
  
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==Lac promoter==
 
==Lac promoter==
 
 
  
 
=lldP=
 
=lldP=

Latest revision as of 11:39, 15 July 2013

Notes on various promoters:

OmpA: Among the strongest promoters in E. coli (1, cited in 2).


Reporter systems

http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html

(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html )


XylS/Pm system

pBAD

  • Inducible by l-arabinose
  • Wild-type E. coli show heterogenous (all-or-nothing) induction upon addition of arabinose.
  • Strains for homogenous induction by arabinose have been developed.
  • Subject to catabolite repression.


Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94830/


Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.: http://jb.asm.org/content/177/14/4121.full

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC345380/


Repression and catabolite gene activation in the araBAD operon. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211852/

Lac promoter

lldP

GreA

Promoter sequences

Description Length (bp) Sequence Location (absolute) Location (relative) Comment
LacUV5 Constitutive promoter
rrnB P1 73 bp fragment gttgcgcggtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccaccact -70 to +3 Strong promoter. Inhibited by ppGpp.
GreA promoter 60bp fragment ggcgcaacgccctataaagtaaacgatgacccttcgggaacttcagggtaaaatgactAt -58 to +2
PcnB
MazEF
IraP
His
Thr


Links/bibliography

http://wolfson.huji.ac.il/expression/vector/promoters.html