User:Jarle Pahr/Kits: Difference between revisions
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Commercial kits for applications in molecular biology: | Commercial kits for applications in molecular biology: | ||
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Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg | Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg | ||
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! Kit!! Use !! Advantages !! Disadvantages !! Time !! Experience | |||
|- | |||
| Promega Wizard SV miniprep || DNA isolation || Quick || Low yield || || | |||
|- | |||
| Machery-Nagel NucleoBond Xtra Midi (midiprep)|| DNA isolation ||High yield (~20 ug) || Slow || 2 d || | |||
|- | |||
| Qiaquick gel extraction|| DNA extraction from gel || Fast|| Impure product || || | |||
|- | |||
| Qiaquick PCR purification|| Purification of PCR products || Fast|| || || | |||
|- | |||
| QIAEX II gel extraction|| DNA extraction from gel || Higher purity than qiaquick || Slower than Quiaquick|| || | |||
|} | |||
Revision as of 06:14, 25 February 2013
Commercial kits for applications in molecular biology:
HisExpress protein purification columns: http://www.claremontbio.com/HisExpress_Protein_Purification_Columns_s/72.htm
(available at http://www.bioquote.com/product.php?id=291680)
PrepEase his-purification: http://www.affymetrix.com/estore/browse/brand/usb/product.jsp?navMode=34000&productId=131366&navAction=jump&aId=productsNav#1_1
Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg
| Kit | Use | Advantages | Disadvantages | Time | Experience |
|---|---|---|---|---|---|
| Promega Wizard SV miniprep | DNA isolation | Quick | Low yield | ||
| Machery-Nagel NucleoBond Xtra Midi (midiprep) | DNA isolation | High yield (~20 ug) | Slow | 2 d | |
| Qiaquick gel extraction | DNA extraction from gel | Fast | Impure product | ||
| Qiaquick PCR purification | Purification of PCR products | Fast | |||
| QIAEX II gel extraction | DNA extraction from gel | Higher purity than qiaquick | Slower than Quiaquick |
